Histrap hp manual

HisTrap HP 1 ml and 5 ml columns are prepacked with Ni Sepharose High Performance, which consists of 34 μm highly cross-linked agarose beads with an immobilized chelating group. The medium has then been charged with Ni2+-ions. Several amino acids, for example histidine, form complexes with many metal ions. Ni Sepharose High Performance. AP 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Water at room temperature 2 The ranges given are estimates based on our knowledge and experience. Please note the following: pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic. HisTrap HP: nickel-charged IMAC for high resolution, his-tagged protein purification µL and 2 mL capillary loops: reproducible, small volume application of sample 50 mL superloop: large volume sample application with minimal sample loss, .

User Manual Edition AB 1 1 Introduction General ÄKTA™prime is a compact, automated liquid chromatography system. It is designed for standard separation applications. ÄKTAprime features: • Easy unpacking and installation. • Application templates ready to use. • Method templates as a basis for creating customized methods. The system is designed to eliminate the manual steps involved in multistep purifications. Traditional methods With ÄKTAxpress When working with tagged proteins Sample preparation Harvest your cells, sonicate, clarify AC1: HisTrap HP, 5ml DS: HiPrep 26/10 Desalting AC2: GSTrap 4B, 1ml DS: HiPrep 26/10 Desalting. 10 Antibody purification. HiTrap Chelating HP 5 × 1 ml HiTrap Chelating HP 1 × 5 ml HiTrap Chelating HP 5 × 5 ml HiTrap Chelating HP × 5 ml* * Special package delivered on specific order. Connectorkit Connectors supplied Usage No. supplied 1/16" male/luer female Connection of syringe to top of HiTrap column 1.

HisTrap FF. HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Change Product. HisTrap ™ HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose ® High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications. • HisTrap™ excel, 1 ml and 5 ml columns prepacked with Ni Sepharose excel. The special type of filters in the top and bottom of HisTrap excel columns makes it possible to directly load cell-free, unclarified samples on the columns without causing back pressure problems. The HisTrap excel columns can be operated with a peristaltic.